Lipase-Catalyzed Transesterification of Egg-Yolk Phophatidylcholine with Concentrate of n-3 Polyunsaturated Fatty Acids from Cod Liver Oil.
Molecules
; 22(10)2017 Oct 19.
Article
em En
| MEDLINE
| ID: mdl-29048366
ABSTRACT
Phospholipids containing PUFAs are important vehicles for their delivering to the targeted tissues. In our research project we established enzymatic methods for the enrichment of natural egg-yolk PC with n-3 PUFAs. Instead of synthetic PUFA ethyl esters, the new strategy was developed using polyunsaturated fatty acids enriched fraction (PUFA-EF) from cod liver oil as the natural acyl donors. PUFA-EF was produced by urea-complexation and contained 86.9% PUFA including 8.5% stearidonic acid (SDA; 184(n-3)), 26.7% EPA, and 45.2% DHA. The transesterification of PC with PUFA was catalyzed by lipases. After screening of enzymes the effect of reaction medium; molar ratio of substrates and etc. was investigated. The highest incorporation of PUFA was 45.6%; including 36.8% DHA and 5.8% EPA at the following reaction conditions hexane; 55 °C; PUFA-EF/PC acyl ratio of 10; 48 h of reaction time and lipase B from Candida antarctica as a biocatalyst (20% of enzyme load).
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Fosfatidilcolinas
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Óleo de Fígado de Bacalhau
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Proteínas Fúngicas
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Ácidos Graxos Ômega-3
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Gema de Ovo
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Lipase
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article