Development of an xTAG-multiplex PCR array for the detection of four avian respiratory viruses.
Mol Cell Probes
; 37: 1-5, 2018 02.
Article
em En
| MEDLINE
| ID: mdl-29054443
ABSTRACT
Acute respiratory tract infections are of paramount importance in the poultry industry. We developed an xTAG bead assay for the simultaneous detection and discrimination of avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV) and infectious laryngotracheitis virus (ILTV). The assay lacked nonspecific reactions with other common avian viruses and the limit of detection was 6.75 × 102- 3.52 × 103copies/µL. We examined 60 clinical specimens and found 18 positive for respiratory viruses. Our result demonstrated that xTAG-multiplex PCR method is a high-throughput, rapid, specific and sensitive assay for use in epidemiological studies and clinical detection of avian respiratory pathogens.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
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Doenças das Aves Domésticas
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Vírus da Doença de Newcastle
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Vírus da Bronquite Infecciosa
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Herpesvirus Galináceo 1
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Reação em Cadeia da Polimerase Multiplex
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article