Effects of TRPC1 on epithelial mesenchymal transition in human airway in chronic obstructive pulmonary disease.
Medicine (Baltimore)
; 96(43): e8166, 2017 Oct.
Article
em En
| MEDLINE
| ID: mdl-29068985
ABSTRACT
BACKGROUND:
We investigated the effects of TRPC1 on epithelial mesenchymal transition (EMT) in human airway in chronic obstructive pulmonary disease (COPD).METHODS:
A total of 94 patients who underwent lobectomy were selected and divided into COPD (49 cases) and control (45 cases) groups. Immunohistochemistry was applied to detect expression of E-cadherin and vimentin and TRPC1. Correlation of TRPC1 expression with E-cadherin and vimentin expression, and correlations of lung function indicators in COPD patients with expression of TRPC1, E-cadherin, and vimentin were analyzed. Human airway epithelial cells (16HBE) were used for cell experiments; and cigarette smoking extract (CSE) was adopted to establish the COPD model using TRPC1 recombinant plasmids and siRNA. Cells were assigned into the control, CSE, CSEâ+âvector, CSEâ+âTRPC1, CSEâ+âsi-NC, and CSEâ+âsi-TRPC1 groups. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were implemented to detect expression of TRPC1, E-cadherin, and vimentin.RESULTS:
Compared with the control group, expression of TRPC1 and vimentin significantly increased while expression of E-cadherin decreased in the COPD group, and protein expression of TRPC1 was positively correlated with the protein expression of vimentin but negatively correlated with the protein expression of E-cadherin. Patients exhibiting positive expression of TRPC1 had lower FEV1, FEV1%Pred, and FEV1/FVC, compared with the patients exhibiting negative expression of TRPC1. Compared with the control group, expression of TRPC1 and vimentin increased, whereas expression of E-cadherin decreased in the CSE, CSEâ+âvector, CSEâ+âTRPC1, and CSEâ+âsi-NC groups. Compared with the CSE and CSEâ+âvector groups, the expression of TRPC1 and vimentin increased but the expression of E-cadherin decreased in the CSEâ+âTRPC1 group. Compared with the CSE and CSEâ+âsi-NC groups, the expression of TRPC1 and vimentin decreased but the expression of E-cadherin increased in the CSEâ+âsi-TRPC1 group. No significant differences were observed among the CSE, CSEâ+âvector and CSEâ+âsi-NC groups.CONCLUSION:
Overexpression of TRPC1 in COPD promoted EMT process and TRPC1 may be a new and interesting focus for COPD new treatment in the future.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doença Pulmonar Obstrutiva Crônica
/
Canais de Cátion TRPC
/
Transição Epitelial-Mesenquimal
Limite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article