Molecular basis for unique specificity of human TRAF4 for platelets GPIbß and GPVI.
Proc Natl Acad Sci U S A
; 114(43): 11422-11427, 2017 10 24.
Article
em En
| MEDLINE
| ID: mdl-29073066
ABSTRACT
Tumor necrosis factor (TNF)-receptor associated factor 4 (TRAF4), an adaptor protein with E3-ligase activity, is involved in embryogenesis, cancer initiation and progression, and platelet receptor (GPIb-IX-V complex and GPVI)-mediated signaling for reactive oxygen species (ROS) production that initiates thrombosis at arterial shears. Disruption of platelet receptors and the TRAF4 interaction is a potential target for therapeutic intervention by antithrombotic drugs. Here, we report a crystal structure of TRAF4 (amino acid residues 290â¼470) in complex with a peptide from the GPIbß receptor (amino acid residues 177â¼181). The GPIbß peptide binds to a unique shallow surface composed of two hydrophobic pockets on TRAF4. Further studies revealed the TRAF4-binding motif Arg-Leu-X-Ala. The TRAF4-binding motif was present not only in platelet receptors but also in the TGF-ß receptor. The current structure will provide a template for furthering our understanding of the receptor-binding specificity of TRAF4, TRAF4-mediated signaling, and related diseases.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Glicoproteínas da Membrana de Plaquetas
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Complexo Glicoproteico GPIb-IX de Plaquetas
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Fator 4 Associado a Receptor de TNF
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article