[Genome-editing: focus on the off-target effects].
Sheng Wu Gong Cheng Xue Bao
; 33(10): 1757-1775, 2017 Oct 25.
Article
em Zh
| MEDLINE
| ID: mdl-29082723
ABSTRACT
Breakthroughs of genome-editing in recent years have paved the way to develop new therapeutic strategies. These genome-editing tools mainly include Zinc-finger nucleases (ZFNs), Transcription activator-like effector nucleases (TALENs), and clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas-based RNA-guided DNA endonucleases. However, off-target effects are still the major issue in genome editing, and limit the application in gene therapy. Here, we summarized the cause and compared different detection methods of off-targets.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Genoma
/
Sistemas CRISPR-Cas
/
Edição de Genes
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2017
Tipo de documento:
Article