Incorporation of a Ligand Peptide for Immune Inhibitory Receptor LAIR-1 on Biomaterial Surfaces Inhibits Macrophage Inflammatory Responses.
Adv Healthc Mater
; 6(24)2017 Dec.
Article
em En
| MEDLINE
| ID: mdl-29083540
ABSTRACT
Leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1) is an inhibitory receptor broadly expressed on immune cells, with its ligands residing within the extracellular matrix protein collagen. In this study, surfaces are modified with a LAIR-1 ligand peptide (LP), and it is observed that macrophages cultured on LAIR-1 LP-conjugated surfaces exhibit significantly reduced secretion of inflammatory cytokines in response to proinflammatory stimuli that reflect an injured environment. These downregulated mediators include TNF-α, MIP-1α, MIP-1ß, MIP-2, RANTES, and MIG. Knockdown of LAIR-1 using siRNA abrogates this inhibition of cytokine secretion, supporting the specificity of the inhibitory effect to this receptor. These results are the first to demonstrate that integration of LAIR-1 ligands with biomaterials could suppress inflammatory responses.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
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Materiais Biocompatíveis
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Receptores Imunológicos
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Macrófagos
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article