Continuous High-Throughput Colorimetric Assays for α-Transaminases.
Methods Mol Biol
; 1685: 233-245, 2018.
Article
em En
| MEDLINE
| ID: mdl-29086312
ABSTRACT
Transaminases are efficient tools for the stereoselective conversion of prochiral ketones into valuable chiral amines. Notably, the diversity of naturally occurring α-transaminases offers access to a wide range of L- and D-α-amino acids. We describe here two continuous colorimetric assays for the quantification of transamination activities between a keto acid and a standard donor substrate (L- or D-Glutamic acid or cysteine sulfinic acid). These assays are helpful for kinetic studies as well as for high-throughput screening of enzyme collections.
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Base de dados:
MEDLINE
Assunto principal:
Ensaios de Triagem em Larga Escala
/
Transaminases
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article