Effects of periconceptional maternal alcohol intake and a postnatal high-fat diet on obesity and liver disease in male and female rat offspring.
Am J Physiol Endocrinol Metab
; 315(4): E694-E704, 2018 10 01.
Article
em En
| MEDLINE
| ID: mdl-29089335
ABSTRACT
The effects of maternal alcohol consumption around the time of conception on offspring are largely unknown and difficult to determine in a human population. This study utilized a rodent model to examine if periconceptional alcohol (PCEtOH) consumption, alone or in combination with a postnatal high-fat diet (HFD), resulted in obesity and liver dysfunction. Sprague-Dawley rats were fed a control or an ethanol-containing [12.5% (vol/vol) EtOH] liquid diet from 4 days before mating until 4 days of gestation ( n = 12/group). A subset of offspring was fed a HFD between 3 and 8 mo of age. In males, PCEtOH and HFD increased total body fat mass ( PPCEtOH < 0.05, PHFD < 0.0001); in females, only HFD increased fat mass ( PHFD < 0.0001). PCEtOH increased microvesicular liver steatosis in male, but not female, offspring. Plasma triglycerides, HDL, and cholesterol were increased in PCEtOH-exposed males ( PPCEtOH < 0.05), and LDL, cholesterol, and leptin (Lep) were increased in PCEtOH-exposed females ( PPCEtOH < 0.05). mRNA levels of Tnf-α and Lep in visceral adipose tissue were increased by PCEtOH in both sexes ( PPCEtOH < 0.05), and Il-6 mRNA was increased in males ( PPCEtOH < 0.05). These findings were associated with reduced expression of microRNA-26a, a known regulator of IL-6 and TNF-α. Alcohol exposure around conception increases obesity risk, alters plasma lipid and leptin profiles, and induces liver steatosis in a sex-specific manner. These programmed phenotypes were similar to those caused by a postnatal HFD, particularly in male offspring. These results have implications for the health of offspring whose mothers consumed alcohol around the time of conception.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Depressores do Sistema Nervoso Central
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Etanol
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Hepatopatia Gordurosa não Alcoólica
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Fígado
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Obesidade
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Pregnancy
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article