MicroRNAs promote skeletal muscle differentiation of mesodermal iPSC-derived progenitors.
Nat Commun
; 8(1): 1249, 2017 11 01.
Article
em En
| MEDLINE
| ID: mdl-29093487
ABSTRACT
Muscular dystrophies (MDs) are often characterized by impairment of both skeletal and cardiac muscle. Regenerative strategies for both compartments therefore constitute a therapeutic avenue. Mesodermal iPSC-derived progenitors (MiPs) can regenerate both striated muscle types simultaneously in mice. Importantly, MiP myogenic propensity is influenced by somatic lineage retention. However, it is still unknown whether human MiPs have in vivo potential. Furthermore, methods to enhance the intrinsic myogenic properties of MiPs are likely needed, given the scope and need to correct large amounts of muscle in the MDs. Here, we document that human MiPs can successfully engraft into the skeletal muscle and hearts of dystrophic mice. Utilizing non-invasive live imaging and selectively induced apoptosis, we report evidence of striated muscle regeneration in vivo in mice by human MiPs. Finally, combining RNA-seq and miRNA-seq data, we define miRNA cocktails that promote the myogenic potential of human MiPs.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Músculo Esquelético
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Desenvolvimento Muscular
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MicroRNAs
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Células-Tronco Pluripotentes Induzidas
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Coração
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Mesoderma
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Distrofia Muscular Animal
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Miocárdio
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article