Detection and differentiation of HIV-2 using the point-of-care Alere q HIV-1/2 Detect nucleic acid test.
J Clin Virol
; 97: 22-25, 2017 12.
Article
em En
| MEDLINE
| ID: mdl-29096389
ABSTRACT
BACKGROUND:
The Alere q HIV-1/2 Detect test (Alere Detect) is a rapid point-of-care (POC) nucleic acid test (NAT) that can detect and differentiate HIV-1 and HIV-2 in 25-µL whole blood or plasma samples. The Alere Detect test has been validated for early infant diagnosis of HIV-1 infection, and it is the only POC NAT device currently known to detect HIV-2, which is endemic in West Africa.OBJECTIVES:
To evaluate the sensitivity detecting HIV-2 RNA and the differential performance of the Alere Detect. STUDYDESIGN:
Plasma samples from non-HIV (n=4), HIV-1 (n=22), HIV-2 (n=111; 29 Group A, 2 Group B) and HIV-1/HIV-2 dually-seropositive (n=8) participants in Senegal and the United States and HIV-2 reference strains (3 Group A, 1 Group B) were tested by Alere Detect, Abbott RealTime HIV-1 and the University of Washington HIV-2 RNA quantitative (UW HIV-2) assays.RESULTS:
The Alere Detect correctly differentiated between HIV-1 and HIV-2 in all 80 (100%) patient samples with detectable HIV RNA (n=20 HIV-1, 60 HIV-2). The overall HIV-2 detection concordance between Alere Detect and the UW HIV-2 assay was 68% (54/80); the concordance improved to 100% (30/30) for samples with HIV-2 RNA >300copies/mL. Neither assay detected HIV-2 RNA in 31 of 111 HIV-2 seropositive samples.CONCLUSIONS:
The Alere Detect test is a novel device detecting HIV RNA in clinical samples, and differentiating HIV-1 and HIV-2 with a high level of specificity. It has the potential for use as a rapid HIV-2 NAT-based diagnosis tool in resource-limited settings and to confirm HIV-2 infection for the CDC 4th generation HIV-1/2 diagnostic algorithm.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Viral
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Infecções por HIV
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HIV-2
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Sistemas Automatizados de Assistência Junto ao Leito
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
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Prognostic_studies
/
Screening_studies
Limite:
Humans
País como assunto:
Africa
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America do norte
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article