Generation of thermostable Moloney murine leukemia virus reverse transcriptase variants using site saturation mutagenesis library and cell-free protein expression system.

Katano, Yuta; Li, Tongyang; Baba, Misato; Nakamura, Miyo; Ito, Masaaki; Kojima, Kenji; Takita, Teisuke; Yasukawa, Kiyoshi

Katano, Yuta; Li, Tongyang; Baba, Misato; Nakamura, Miyo; Ito, Masaaki; Kojima, Kenji; Takita, Teisuke; Yasukawa, Kiyoshi.

Afiliação

Katano Y; a Division of Food Science and Biotechnology, Graduate School of Agriculture , Kyoto University , Kyoto , Japan.
Li T; a Division of Food Science and Biotechnology, Graduate School of Agriculture , Kyoto University , Kyoto , Japan.
Baba M; a Division of Food Science and Biotechnology, Graduate School of Agriculture , Kyoto University , Kyoto , Japan.
Nakamura M; a Division of Food Science and Biotechnology, Graduate School of Agriculture , Kyoto University , Kyoto , Japan.
Ito M; b Department of Bioresources Engineering , National Institute of Technology, Okinawa College , Nago , Japan.
Kojima K; a Division of Food Science and Biotechnology, Graduate School of Agriculture , Kyoto University , Kyoto , Japan.
Takita T; a Division of Food Science and Biotechnology, Graduate School of Agriculture , Kyoto University , Kyoto , Japan.
Yasukawa K; a Division of Food Science and Biotechnology, Graduate School of Agriculture , Kyoto University , Kyoto , Japan.

Biosci Biotechnol Biochem ; 81(12): 2339-2345, 2017 Dec.

Article em En | MEDLINE | ID: mdl-29103348

Assuntos

Escherichia coli/genética; Vírus da Leucemia Murina de Moloney/enzimologia; Mutagênese; Mutação; DNA Polimerase Dirigida por RNA/química; DNA Polimerase Dirigida por RNA/genética; Temperatura; Animais; Estabilidade Enzimática/genética; Expressão Gênica; Camundongos; Modelos Moleculares; Vírus da Leucemia Murina de Moloney/genética; Conformação Proteica; DNA Polimerase Dirigida por RNA/metabolismo

Palavras-chave

Moloney murine leukemia virus; cell-free protein expression; reverse transcriptase; site saturation mutagenesis library; thermostability

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Temperatura / Mutagênese / DNA Polimerase Dirigida por RNA / Escherichia coli / Vírus da Leucemia Murina de Moloney / Mutação Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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