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Association between a MIR499A polymorphism and diabetic neuropathy in type 2 diabetes.
Ciccacci, Cinzia; Latini, Andrea; Greco, Carla; Politi, Cristina; D'Amato, Cinzia; Lauro, Davide; Novelli, Giuseppe; Borgiani, Paola; Spallone, Vincenza.
Afiliação
  • Ciccacci C; Department of Biomedicine and Prevention, Genetics Section, University of Rome "Tor Vergata", Italy.
  • Latini A; Department of Biomedicine and Prevention, Genetics Section, University of Rome "Tor Vergata", Italy.
  • Greco C; Department of Systems Medicine, Endocrinology, University of Rome "Tor Vergata", Italy.
  • Politi C; Department of Biomedicine and Prevention, Genetics Section, University of Rome "Tor Vergata", Italy.
  • D'Amato C; Department of Systems Medicine, Endocrinology, University of Rome "Tor Vergata", Italy.
  • Lauro D; Department of Systems Medicine, Endocrinology, University of Rome "Tor Vergata", Italy.
  • Novelli G; Department of Biomedicine and Prevention, Genetics Section, University of Rome "Tor Vergata", Italy.
  • Borgiani P; Department of Biomedicine and Prevention, Genetics Section, University of Rome "Tor Vergata", Italy. Electronic address: borgiani@med.uniroma2.it.
  • Spallone V; Department of Systems Medicine, Endocrinology, University of Rome "Tor Vergata", Italy.
J Diabetes Complications ; 32(1): 11-17, 2018 01.
Article em En | MEDLINE | ID: mdl-29108839
AIMS: Diabetic polyneuropathy (DPN) and cardiovascular autonomic neuropathy (CAN) affect a large percentage of diabetic people and impact severely on quality of life. As it seems that miRNAs and their variations might play a role in these complications, we investigated whether the rs3746444 SNP in the MIR499A gene could be associated with susceptibility to DPN and/or CAN. METHODS: We analyzed 150 participants with type 2 diabetes. DNA was extracted from peripheral blood samples and genotyping was performed by TaqMan genotyping assay. Cardiovascular tests, MNSI-Q and MDNS for neuropathic symptoms and signs, VPT, and thermal thresholds were used for CAN and DPN assessment. We performed a genotype-phenotype correlation analysis. RESULTS: We observed that the GG genotype was associated with a higher risk of developing CAN (P=0.002 and OR=16.08, P=0.0005 and OR=35.02, for early and confirmed CAN, respectively) and DPN (P=0.037 and OR=6.56), after correction for BMI, sex, age, HbA1c and disease duration. Moreover, the GG genotype was associated with worse values of MDNS (P=0.017), VPT (P=0.01), thermal thresholds (P=0.01), and CAN score (P<0.001). A logistic multivariate analysis confirmed that MIR499A GG genotype, disease duration and HbA1c contributed to early CAN (R2=0.26), while the same variables and age contributed to DPN (R2=0.21). With a multiple linear regression, we observed that GG genotype (P=0.001) and disease duration (P=0.035) were the main variables contributing to the CAN score (R2=0.35). CONCLUSIONS: We described for the first time that the MIR499A genetic variation could be involved in diabetic neuropathies susceptibility. In particular, patients carrying the rs3746444 GG genotype had a higher risk of CAN development, together with a more severe form of CAN.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polimorfismo de Nucleotídeo Único / MicroRNAs / Diabetes Mellitus Tipo 2 / Neuropatias Diabéticas Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polimorfismo de Nucleotídeo Único / MicroRNAs / Diabetes Mellitus Tipo 2 / Neuropatias Diabéticas Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2018 Tipo de documento: Article