Cloning and Expression of Novel Aminoglycoside Phosphotransferase Genes from Campylobacter and Their Role in the Resistance to Six Aminoglycosides.
Antimicrob Agents Chemother
; 62(1)2018 01.
Article
em En
| MEDLINE
| ID: mdl-29109167
ABSTRACT
Nine aph genes, including aph(2â³)-Ib, aph(2â³)-Ic, aph(2â³)-Ig, aph(2â³)-If, aph(2â³)-If1, aph(2â³)-If3, aph(2â³)-Ih, aac(6')-Ie-aph(2â³)-Ia, and aac(6')-Ie-aph(2â³)-If2, were previously identified in Campylobacter To measure the contribution of these alleles to aminoglycoside resistance, we cloned nine genes into the pBluescript and expressed them in Escherichia coli DH5α. The nine aph expressed in E. coli showed various levels of resistance to gentamicin, kanamycin, and tobramycin. Three genes, aac(6â³)-Ie-aph(2â³)-Ia, aph2â³-If1, and aph2â³-Ig, showed increased MICs to amikacin, and five aph genes were transferrable.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Campylobacter
/
Canamicina Quinase
/
Farmacorresistência Bacteriana
/
Aminoglicosídeos
/
Antibacterianos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article