Structure and function of yeast Atg20, a sorting nexin that facilitates autophagy induction.
Proc Natl Acad Sci U S A
; 114(47): E10112-E10121, 2017 11 21.
Article
em En
| MEDLINE
| ID: mdl-29114050
ABSTRACT
The Atg20 and Snx4/Atg24 proteins have been identified in a screen for mutants defective in a type of selective macroautophagy/autophagy. Both proteins are connected to the Atg1 kinase complex, which is involved in autophagy initiation, and bind phosphatidylinositol-3-phosphate. Atg20 and Snx4 contain putative BAR domains, suggesting a possible role in membrane deformation, but they have been relatively uncharacterized. Here we demonstrate that, in addition to its function in selective autophagy, Atg20 plays a critical role in the efficient induction of nonselective autophagy. Atg20 is a dynamic posttranslationally modified protein that engages both structurally stable (PX and BAR) and intrinsically disordered domains for its function. In addition to its PX and BAR domains, Atg20 uses a third membrane-binding module, a membrane-inducible amphipathic helix present in a previously undescribed location in Atg20 within the putative BAR domain. Taken together, these findings yield insights into the molecular mechanism of the autophagy machinery.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Autofagia
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Regulação Fúngica da Expressão Gênica
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Proteínas de Saccharomyces cerevisiae
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Nexinas de Classificação
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Proteínas Relacionadas à Autofagia
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article