Nano/Micro Formulations for Bacteriophage Delivery.
Methods Mol Biol
; 1693: 271-283, 2018.
Article
em En
| MEDLINE
| ID: mdl-29119446
ABSTRACT
Encapsulation methodologies allow the protection of bacteriophages for overcoming critical environmental conditions. Moreover, they improve the stability and the controlled delivery of bacteriophages which is of great innovative value in bacteriophage therapy. Here, two different encapsulation methodologies of bacteriophages are described using two biocompatible materials a lipid cationic mixture and a combination of alginate with the antacid CaCO3. To perform bacteriophage encapsulation, a purified lysate highly concentrated (around 1010-1011 pfu/mL) is necessary, and to dispose of a specific equipment. Both methodologies have been successfully applied for encapsulating Salmonella bacteriophages with different morphologies. Also, the material employed does not modify the antibacterial action of bacteriophages. Moreover, both technologies can also be adapted to any bacteriophage and possibly to any delivery route for bacteriophage therapy.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Bacteriófagos
/
Composição de Medicamentos
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Terapia por Fagos
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Antibacterianos
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article