Cohesin Ubiquitylation and Mobilization Facilitate Stalled Replication Fork Dynamics.
Mol Cell
; 68(4): 758-772.e4, 2017 Nov 16.
Article
em En
| MEDLINE
| ID: mdl-29129641
ABSTRACT
Replication fork integrity is challenged in conditions of stress and protected by the Mec1/ATR checkpoint to preserve genome stability. Still poorly understood in fork protection is the role played by the structural maintenance of chromosomes (SMC) cohesin complex. We uncovered a role for the Rsp5Bul2 ubiquitin ligase in promoting survival to replication stress by preserving stalled fork integrity. Rsp5Bul2 physically interacts with cohesin and the Mec1 kinase, thus promoting checkpoint-dependent cohesin ubiquitylation and cohesin-mediated fork protection. Ubiquitylation mediated by Rsp5Bul2 promotes cohesin mobilization from chromatin neighboring stalled forks, likely by stimulating the Cdc48/p97 ubiquitin-selective segregase, and its timely association to nascent chromatids. This Rsp5Bul2 fork protection mechanism requires the Wpl1 cohesin mobilizer as well as the function of the Eco1 acetyltransferase securing sister chromatid entrapment. Our data indicate that ubiquitylation facilitates cohesin dynamic interfacing with replication forks within a mechanism preserving stalled-fork functional architecture.
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MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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DNA Fúngico
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Proteínas Cromossômicas não Histona
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Proteínas de Ciclo Celular
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Replicação do DNA
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Ubiquitinação
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article