Preparation of a beta-tricalcium phosphate nanocoating and its protein adsorption behaviour by quartz crystal microbalance with dissipation technique.
Colloids Surf B Biointerfaces
; 162: 1-7, 2018 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-29132041
ABSTRACT
Beta-tricalcium phosphate (ß-TCP) nanocoatings, which can be analysed using a quartz crystal microbalance with dissipation technique (QCM-D), were fabricated on a gold surface by electrophoretic deposition. The influences of electric field intensity and electrophoresis time were investigated. The adsorption behaviours of bovine serum albumin (BSA) and lysozyme (LSZ) on Au and ß-TCP surfaces were observed in real time by QCM-D. The homogeneous ß-TCP nanocoating with moderately sized particles on gold surface was fabricated at 25V/cm for 5min, and it met the requirements for the QCM-D experiment. The adsorption behaviour of BSA was different from that of LSZ, which was caused by the differences of protein properties. The adsorption quantity of BSA on a ß-TCP surface was higher than that on a gold surface. However, the adsorption amount of LSZ on a ß-TCP surface was lower than that on a gold surface. The electrostatic force was the major factor affecting the adsorption quantities of BSA and LSZ on Au and ß-TCP surfaces based on the investigation of various factors. The findings reported here will be useful for understanding the mechanism of the interaction between biomaterials and proteins.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Fosfatos de Cálcio
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Soroalbumina Bovina
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Muramidase
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Nanoestruturas
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Técnicas de Microbalança de Cristal de Quartzo
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Ouro
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article