Differential Immunodominance Hierarchy of CD8+ T-Cell Responses in HLA-B*27:05- and -B*27:02-Mediated Control of HIV-1 Infection.
J Virol
; 92(4)2018 02 15.
Article
em En
| MEDLINE
| ID: mdl-29167337
ABSTRACT
The well-characterized association between HLA-B*2705 and protection against HIV disease progression has been linked to immunodominant HLA-B*2705-restricted CD8+ T-cell responses toward the conserved Gag KK10 (residues 263 to 272) and polymerase (Pol) KY9 (residues 901 to 909) epitopes. We studied the impact of the 3 amino acid differences between HLA-B*2705 and the closely related HLA-B*2702 on the HIV-specific CD8+ T-cell response hierarchy and on immune control of HIV. Genetic epidemiological data indicate that both HLA-B*2702 and HLA-B*2705 are associated with slower disease progression and lower viral loads. The effect of HLA-B*2702 appeared to be consistently stronger than that of HLA-B*2705. In contrast to HLA-B*2705, the immunodominant HIV-specific HLA-B*2702-restricted CD8+ T-cell response is to a Nef epitope (residues 142 to 150 [VW9]), with Pol KY9 subdominant and Gag KK10 further subdominant. This selection was driven by structural differences in the F pocket, mediated by a polymorphism between these two HLA alleles at position 81. Analysis of autologous virus sequences showed that in HLA-B*2702-positive subjects, all three of these CD8+ T-cell responses impose selection pressure on the virus, whereas in HLA-B*2705-positive subjects, there is no Nef VW9-mediated selection pressure. These studies demonstrate that HLA-B*2702 mediates protection against HIV disease progression that is at least as strong as or stronger than that mediated by HLA-B*2705. In combination with the protective Gag KK10 and Pol KY9 CD8+ T-cell responses that dominate HIV-specific CD8+ T-cell activity in HLA-B*2705-positive subjects, a Nef VW9-specific response is additionally present and immunodominant in HLA-B*2702-positive subjects, mediated through a polymorphism at residue 81 in the F pocket, that contributes to selection pressure against HIV.IMPORTANCE CD8+ T cells play a central role in successful control of HIV infection and have the potential also to mediate the eradication of viral reservoirs of infection. The principal means by which protective HLA class I molecules, such as HLA-B*2705 and HLA-B*5701, slow HIV disease progression is believed to be via the particular HIV-specific CD8+ T cell responses restricted by those alleles. We focus here on HLA-B*2705, one of the best-characterized protective HLA molecules, and the closely related HLA-B*2702, which differs by only 3 amino acids and which has not been well studied in relation to control of HIV infection. We show that HLA-B*2702 is also protective against HIV disease progression, but the CD8+ T-cell immunodominance hierarchy of HLA-B*2702 differs strikingly from that of HLA-B*2705. These findings indicate that the immunodominant HLA-B*2702-restricted Nef response adds to protection mediated by the Gag and Pol specificities that dominate anti-HIV CD8+ T-cell activity in HLA-B*2705-positive subjects.
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MEDLINE
Assunto principal:
Infecções por HIV
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Antígeno HLA-B27
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Epitopos Imunodominantes
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Linfócitos T CD8-Positivos
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Produtos do Gene gag do Vírus da Imunodeficiência Humana
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Produtos do Gene nef do Vírus da Imunodeficiência Humana
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article