Graphene oxide induces cytotoxicity and oxidative stress in bluegill sunfish cells.
J Appl Toxicol
; 38(4): 504-513, 2018 04.
Article
em En
| MEDLINE
| ID: mdl-29171043
ABSTRACT
Graphene oxide (GO) is considered a promising material for biological application due to its unique properties. However, the potential toxicity of GO to aquatic organism particularly bluegill sun fish cells (BF-2) is unexplored or remains poorly understood. GO-induced cytotoxicity and oxidative stress in BF-2 cells were assessed using a battery of biomarkers. Two different biological assays (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and neutral red uptake were used to evaluate the cytotoxicity of GO on BF-2 cells. It was found that GO induced dose- and time-dependent cytotoxicity on BF-2 cells. BF-2 cells exposed to lower concentration of GO (40 µg ml-1 ) for 24 induced morphological changes when compared to their respective controls. As evidence for oxidative stress lipid peroxidation, superoxide dismutase, catalase, reactive oxygen species and 8-hydroxy-2'-deoxyguanosine levels were increased and glutathione levels were found to decline in BF-2 cells after treatment with GO. Our findings demonstrate that GO when exposed to BF-2 fish cells cause oxidative stress.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Perciformes
/
Estresse Oxidativo
/
Grafite
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article