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Rational engineering of a native hyperthermostable lactonase into a broad spectrum phosphotriesterase.
Jacquet, Pauline; Hiblot, Julien; Daudé, David; Bergonzi, Céline; Gotthard, Guillaume; Armstrong, Nicholas; Chabrière, Eric; Elias, Mikael.
Afiliação
  • Jacquet P; CNRS UMR 7278, IRD198, INSERM U1095, APHM, Institut Hospitalier Universitaire Méditerranée-Infection, Aix-Marseille Université, 19-21 Bd Jean Moulin, 13005, Marseille, France.
  • Hiblot J; CNRS UMR 7278, IRD198, INSERM U1095, APHM, Institut Hospitalier Universitaire Méditerranée-Infection, Aix-Marseille Université, 19-21 Bd Jean Moulin, 13005, Marseille, France.
  • Daudé D; MPI for Medical Research, Chemical Biology department (EPFL), Heidelberg, Germany.
  • Bergonzi C; Gene&GreenTK, IHU Méditerranée Infection, 19-21 Bd Jean Moulin, 13005, Marseille, France.
  • Gotthard G; CNRS UMR 7278, IRD198, INSERM U1095, APHM, Institut Hospitalier Universitaire Méditerranée-Infection, Aix-Marseille Université, 19-21 Bd Jean Moulin, 13005, Marseille, France.
  • Armstrong N; University of Minnesota, Department of Biochemistry, Molecular Biology and Biophysics & Biotechnology Institute, St. Paul, MN, 55108, USA.
  • Chabrière E; CNRS UMR 7278, IRD198, INSERM U1095, APHM, Institut Hospitalier Universitaire Méditerranée-Infection, Aix-Marseille Université, 19-21 Bd Jean Moulin, 13005, Marseille, France.
  • Elias M; CNRS UMR 7278, IRD198, INSERM U1095, APHM, Institut Hospitalier Universitaire Méditerranée-Infection, Aix-Marseille Université, 19-21 Bd Jean Moulin, 13005, Marseille, France.
Sci Rep ; 7(1): 16745, 2017 12 01.
Article em En | MEDLINE | ID: mdl-29196634
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Engenharia de Proteínas / Hidrolases de Triester Fosfórico Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Engenharia de Proteínas / Hidrolases de Triester Fosfórico Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2017 Tipo de documento: Article