Synthesis of glycoconjugates derived from various lipopolysaccharides of the Vibrionaceae family.
Eur J Biochem
; 179(3): 651-7, 1989 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-2920731
ABSTRACT
Conjugation of simple ketoses (such as 3-deoxy-D-manno-2-octulosonic acid and N-acetylneuraminic acid) and of various O-specific polysaccharides (from Aeromonas hydrophila and Aeromonas salmonicida) to the bifunctional spacer 1,6-hexanediamine, was achieved by reductive amination. The saccharide--1-(6-amino)-hexane alkyamines obtained were converted into the corresponding isothiocyanate derivatives and coupled to the free epsilon-amino group of lysine residues of the protein carrier bovine serum albumin. In similar manner, the aldehyde group introduced by selective periodate oxidation into the partially O-deacylated lipopolysaccharide of Vibrio anguillarum was conjugated to 1,6-hexanediamine, converted into the corresponding isothiocyanate and covalently attached to bovine serum albumin.
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Base de dados:
MEDLINE
Assunto principal:
Vibrio
/
Vibrionaceae
/
Glicoconjugados
/
Lipopolissacarídeos
Idioma:
En
Ano de publicação:
1989
Tipo de documento:
Article