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Multiplexed targeted mass spectrometry assays for prostate cancer-associated urinary proteins.
Shi, Tujin; Quek, Sue-Ing; Gao, Yuqian; Nicora, Carrie D; Nie, Song; Fillmore, Thomas L; Liu, Tao; Rodland, Karin D; Smith, Richard D; Leach, Robin J; Thompson, Ian M; Vitello, Elizabeth A; Ellis, William J; Liu, Alvin Y; Qian, Wei-Jun.
Afiliação
  • Shi T; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Quek SI; Department of Urology, University of Washington, Seattle, WA, USA.
  • Gao Y; Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA.
  • Nicora CD; Present address: Singapore Polytechnic, Center for Biomedical and Life Sciences T11A-412 (level 4), Singapore.
  • Nie S; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Fillmore TL; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Liu T; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Rodland KD; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Smith RD; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Leach RJ; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Thompson IM; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Vitello EA; Department of Urology and the Cancer Therapy and Research Center, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
  • Ellis WJ; Department of Urology and the Cancer Therapy and Research Center, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
  • Liu AY; Department of Urology, University of Washington, Seattle, WA, USA.
  • Qian WJ; Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA.
Oncotarget ; 8(60): 101887-101898, 2017 Nov 24.
Article em En | MEDLINE | ID: mdl-29254211
ABSTRACT
Biomarkers for effective early diagnosis and prognosis of prostate cancer are still lacking. Multiplexed assays for cancer-associated proteins could be useful for identifying biomarkers for cancer detection and stratification. Herein, we report the development of sensitive targeted mass spectrometry assays for simultaneous quantification of 10 prostate cancer-associated proteins in urine. The diagnostic utility of these markers was evaluated with an initial cohort of 20 clinical urine samples. Individual marker concentration was normalized against the measured urinary prostate-specific antigen level as a reference of prostate-specific secretion. The areas under the receiver-operating characteristic curves for the 10 proteins ranged from 0.75 for CXL14 to 0.87 for CEAM5. Furthermore, MMP9 level was found to be significantly higher in patients with high Gleason scores, suggesting a potential of MMP9 as a marker for risk level assessment. Taken together, our work illustrated the feasibility of accurate multiplexed measurements of low-abundance cancer-associated proteins in urine and provided a viable path forward for preclinical verification of candidate biomarkers for prostate cancer.
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Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Prognostic_studies / Risk_factors_studies / Screening_studies Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Prognostic_studies / Risk_factors_studies / Screening_studies Idioma: En Ano de publicação: 2017 Tipo de documento: Article