Rapid Analysis of Unsaturated Fatty Acids on Paper-Based Analytical Devices via Online Epoxidation and Ambient Mass Spectrometry.
Anal Chem
; 90(3): 2070-2078, 2018 02 06.
Article
em En
| MEDLINE
| ID: mdl-29272100
ABSTRACT
In this work, we demonstrate a novel design that allows rapid online identification and quantitation of unsaturated fatty acid CâC location isomers via epoxidation and ambient mass spectrometry (MS). Unsaturated fatty acid solution was loaded on a paper strip placed between a low-temperature plasma probe and the inlet of a mass spectrometer. Reactive oxygen species in the plasma promoted epoxidation at the CâC, and the product was simultaneously ionized. Upon collision-induced dissociation (CID), the epoxidation product was fragmented to release diagnostic ions specific to the CâC location. The whole analytical workflow can be completed within 5 s and is particularly promising for point-of-care (POC) clinical diagnosis, considering its fast, high-throughput nature, and coupling with paper-based analytical devices.
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MEDLINE
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En
Ano de publicação:
2018
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Article