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Exploring the Interaction Between eIF2α Dysregulation, Acute Endoplasmic Reticulum Stress and DYT1 Dystonia in the Mammalian Brain.
Beauvais, Genevieve; Rodriguez-Losada, Noela; Ying, Lei; Zakirova, Zuchra; Watson, Jaime L; Readhead, Ben; Gadue, Paul; French, Deborah L; Ehrlich, Michelle E; Gonzalez-Alegre, Pedro.
Afiliação
  • Beauvais G; Raymond G. Perelman Center for Cellular & Molecular Therapeutics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, United States.
  • Rodriguez-Losada N; Department of Human Physiology, University of Malaga, Malaga 29071, Spain.
  • Ying L; Raymond G. Perelman Center for Cellular & Molecular Therapeutics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, United States.
  • Zakirova Z; Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States.
  • Watson JL; Raymond G. Perelman Center for Cellular & Molecular Therapeutics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, United States.
  • Readhead B; Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States.
  • Gadue P; Raymond G. Perelman Center for Cellular & Molecular Therapeutics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, United States; Department of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, United States.
  • French DL; Raymond G. Perelman Center for Cellular & Molecular Therapeutics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, United States; Department of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, United States.
  • Ehrlich ME; Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States.
  • Gonzalez-Alegre P; Raymond G. Perelman Center for Cellular & Molecular Therapeutics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, United States; Department of Neurology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, United States. Electronic address: ped
Neuroscience ; 371: 455-468, 2018 02 10.
Article em En | MEDLINE | ID: mdl-29289717
ABSTRACT
DYT1 dystonia is a neurological disease caused by dominant mutations in the TOR1A gene, encoding for the endoplasmic reticulum (ER)-resident protein torsinA. Recent reports linked expression of the DYT1-causing protein with dysregulation of eIF2α, a key component of the cellular response to ER stress known as the unfolded protein response (UPR). However, the response of the DYT1 mammalian brain to acute ER stress inducers has not been evaluated in vivo. We hypothesized that torsinA regulates the neuronal UPR and expression of its mutant form would alter this process. TorsinA was post-transcriptionally upregulated upon acute ER stress in different models, suggesting a role in this response. Moreover, increased basal phosphorylation of eIF2α in DYT1 transgenic rats was associated with an abnormal response to acute ER stress. Finally, an unbiased RNA-Seq-based transcriptomic analysis of embryonic brain tissue in heterozygous and homozygous DYT1 knockin mice confirmed the presence of eIF2α dysregulation in the DYT1 brain. In sum, these findings support previous reports linking torsinA function, eIF2α signaling and the neuronal response to ER stress in vivo. Furthermore, we describe novel protocols to investigate neuronal ER stress in cultured neurons and in vivo.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Encéfalo / Fator de Iniciação 2 em Eucariotos / Distonia Muscular Deformante / Estresse do Retículo Endoplasmático Tipo de estudo: Prognostic_studies Limite: Animals / Female / Humans / Male Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Encéfalo / Fator de Iniciação 2 em Eucariotos / Distonia Muscular Deformante / Estresse do Retículo Endoplasmático Tipo de estudo: Prognostic_studies Limite: Animals / Female / Humans / Male Idioma: En Ano de publicação: 2018 Tipo de documento: Article