Effect of indomethacin and benoxaprofen on immune complex interaction with cultured glomerular cells.

ABSTRACT

Prostaglandins of the E series (PGE) increased immune complex (IC) interaction with cultured glomerular cells in a previous study. The present study examines the effect of indomethacin (IND) and benoxaprofen (BEN) on interaction of IC with cultured cells and their effect on PGE enhanced cell-IC interaction. IC were formed with antigen modified to produce a cationic (CAT) charge or left unmodified (UM). IND increased cell interaction with IC formed with CAT antigen (CAT IC). BEN had no effect on the interaction of IC formed with either antigen. The combined use of IND and PGE increased CAT IC interaction to the same degree as when each was used alone. BEN prevented the increased CAT IC interaction produced by IND or PGE when used in combination. Protamine sulfate prevented the enhanced CAT IC interaction produced by IND or PGE while sodium heparin had no effect. The results indicate IND and PGE increase cell IC interaction, the increase is not additive when they are combined, the effects of both are blocked by BEN, and protamine sulfate inhibits the effects of both compounds.