A Comprehensive Protocol Resource for Performing Pooled shRNA and CRISPR Screens.
Methods Mol Biol
; 1725: 201-227, 2018.
Article
em En
| MEDLINE
| ID: mdl-29322420
ABSTRACT
This chapter details a compendium of protocols that collectively enable the reader to perform a pooled shRNA and/or CRISPR screen-with methods to identify and validate positive controls and subsequent hits; establish a viral titer in the cell line of choice; create and screen libraries, sequence strategies, and bioinformatics resources to analyze outcomes. Collectively, this provides an overarching resource from the start to finish of a screening project, making this technology possible in all laboratories.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Biblioteca Gênica
/
Lentivirus
/
RNA Interferente Pequeno
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Ensaios de Triagem em Larga Escala
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Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article