Structural characteristics and anticancer activity in vitro of fucoidan from brown alga Padina boryana.
Carbohydr Polym
; 184: 260-268, 2018 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-29352918
ABSTRACT
The sulfated and acetylated fucoidan fraction, containing fucose, galactose, mannose, glucose and uronic acid residues, was isolated from the brown alga Padina boryana. The structure of galactofucan part was studied after different modifications by NMR spectroscopy and mass spectrometry. It was shown that galactofucan contained the main chain of alternating 1,4-linked α-l-fucopyranose and 1,3-linked ß-d-Galactopyranose. Single fucose residues were found as branches at C4 of galactose residues. Also, fucoidan contained 1,3- or 1,4-linked Fuc-Fuc and Gal-Gal fragments. The sulfate groups occupied positions C2, C3 and C4 of both fucose and galactose residues, which was shown by tandem mass spectrometry of fragments, labeled with heavy-oxygen. The anticancer effect of native and modified fucoidan fractions was studied in vitro on the colorectal carcinoma cells DLD-1 and HCT-116. All fucoidans had no cytotoxicity under 400⯵g/mL and inhibited colony formation of cancer cells at concentration of 200⯵g/mL.
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MEDLINE
Assunto principal:
Polissacarídeos
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Animals
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Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article