Multiscale Structuring of the E. coli Chromosome by Nucleoid-Associated and Condensin Proteins.
Cell
; 172(4): 771-783.e18, 2018 02 08.
Article
em En
| MEDLINE
| ID: mdl-29358050
ABSTRACT
As in eukaryotes, bacterial genomes are not randomly folded. Bacterial genetic information is generally carried on a circular chromosome with a single origin of replication from which two replication forks proceed bidirectionally toward the opposite terminus region. Here, we investigate the higher-order architecture of the Escherichia coli genome, showing its partition into two structurally distinct entities by a complex and intertwined network of contacts the replication terminus (ter) region and the rest of the chromosome. Outside of ter, the condensin MukBEF and the ubiquitous nucleoid-associated protein (NAP) HU promote DNA contacts in the megabase range. Within ter, the MatP protein prevents MukBEF activity, and contacts are restricted to â¼280 kb, creating a domain with distinct structural properties. We also show how other NAPs contribute to nucleoid organization, such as H-NS, which restricts short-range interactions. Combined, these results reveal the contributions of major evolutionarily conserved proteins in a bacterial chromosome organization.
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Base de dados:
MEDLINE
Assunto principal:
Cromossomos Bacterianos
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Adenosina Trifosfatases
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Escherichia coli K12
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Complexos Multiproteicos
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Proteínas de Ligação a DNA
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article