MicroRNA539 inhibits the proliferation and invasion of bladder cancer cells by regulating IGF1R.
Mol Med Rep
; 17(4): 4917-4924, 2018 04.
Article
em En
| MEDLINE
| ID: mdl-29393438
ABSTRACT
Bladder cancer is the most frequent malignancy of the urinary tract and the seventh most common cancer worldwide. The abnormal expression of microRNAs has been frequently observed in various types of human cancers, including bladder cancer. In addition, an increasing body of evidence has demonstrated that microRNAs are potential targets for cancer diagnosis, treatments and prognosis. The aim of the present study was to investigate the expression patterns and potential roles of microRNA539 (miR539) in bladder cancer and its underlying mechanism. Reverse transcriptionquantitative polymerase chain reaction (RTqPCR) was performed to detect miR539 expression in the bladder cancer tissues and cell lines. Following transfection, MTT and cell invasion assays were used to investigate the effects of miR539 overexpression or IGF1R underexpression on bladder cancer cell proliferation and invasion. Bioinformatics analysis, a luciferase reporter assay, RTqPCR and western blot analysis were utilized to determine the potential targets of miR539 in bladder cancer. The results revealed that miR539 levels were relatively decreased in bladder cancer tissues and cell lines when compared with those observed in the matched adjacent normal bladder tissues and normal bladder epithelial cell line. miR539 expression was associated with the tumor stage and lymph node metastasis of patients with bladder cancer. In addition, the expression of miR539 suppressed bladder cancer cell proliferation and invasion. Insulin like growth factor 1 receptor (IGF1R) was identified as a direct target of miR539, and miR539 was also observed to regulate the protein kinase B and extracellular signalregulated kinases signaling pathways. IGF1R was markedly upregulated in bladder cancer tissues and negatively associated with miR539 expression levels. Furthermore, IGF1R knockdown in bladder cancer cells significantly inhibited cell proliferation and invasion. To the best of our knowledge, these results demonstrated for the first time that miR539 may act as a tumor suppressor and serve important roles in tumorigenesis and progression of bladder cancer. Thus, miR539/IGF1R may be a potential therapeutic target for the treatment of bladder cancer.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Bexiga Urinária
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Regulação Neoplásica da Expressão Gênica
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Receptores de Somatomedina
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MicroRNAs
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Interferência de RNA
Tipo de estudo:
Prognostic_studies
Limite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article