Increased expression of angiogenic cytokines in CD56+ uterine natural killer cells from women with recurrent miscarriage.
Cytokine
; 110: 272-276, 2018 10.
Article
em En
| MEDLINE
| ID: mdl-29396049
ABSTRACT
OBJECTIVE:
To compare the expression pattern of angiogenic cytokines in CD56+ uNK cells from peri-implantation endometrium in women with a history of recurrent miscarriage and fertile controls.METHODS:
28 women were recruited, from which 18 women were diagnosed as recurrent miscarriage and 10 women were of proven fertility. Endometrial biopsy samples were obtained precisely 7â¯days after luteinization hormone surge in a natural cycle. The angiogenic profile of isolated CD56+ uNK cells was determined by RayBio human angiogenesis antibody array G Series 1000. Differentially expressed angiogenic cytokines between groups were validated by ELISA kits.RESULTS:
CD56+ uNK cells freshly isolated from peri-implantation endometrium were determined to be >90% pure. Angiogenic cytokine array demonstrated that CD56+ uNK cells are one of the angiogenic factors producers in endometrium around the time of embryo implantation. A differential angiogenic cytokine expression profile was found between two groups, with significantly higher expressions of angiogenin, VEGF-A and bFGF in CD56+ uNK cells from women with recurrent miscarriage, compared with fertile controls.CONCLUSIONS:
Differential angiogenic cytokine profile of isolated CD56+ uNK cells suggested the role of uNK cells in the altered endometrial vascularity at the time of implantation, which may account for the endometrial contribution to recurrent miscarriage.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Útero
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Células Matadoras Naturais
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Aborto Habitual
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Citocinas
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Antígeno CD56
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Indutores da Angiogênese
Limite:
Adult
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Female
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Humans
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Pregnancy
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article