Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures.
J Zhejiang Univ Sci B
; 19(2): 159-167, 2018.
Article
em En
| MEDLINE
| ID: mdl-29405043
ABSTRACT
To address the controversial issue of the toxicity of dental alloys and silver nanoparticles in medical applications, an in vivo-like LO2 3-D model was constructed within polyvinylidene fluoride hollow fiber materials to mimic the microenvironment of liver tissue. The use of microscopy methods and the measurement of liver-specific functions optimized the model for best cell performances and also proved the superiority of the 3-D LO2 model when compared with the traditional monolayer model. Toxicity tests were conducted using the newly constructed model, finding that four dental castings coated with silver nanoparticles were toxic to human hepatocytes after cell viability assays. In general, the toxicity of both the castings and the coated silver nanoparticles aggravated as time increased, yet the nanoparticles attenuated the general toxicity by preventing metal ion release, especially at high concentrations.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Prata
/
Técnica de Fundição Odontológica
/
Hepatócitos
/
Nanopartículas Metálicas
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article