Delivery of adipose-derived stem cells in poloxamer hydrogel improves peripheral nerve regeneration.
Muscle Nerve
; 58(2): 251-260, 2018 Aug.
Article
em En
| MEDLINE
| ID: mdl-29406624
ABSTRACT
INTRODUCTION:
Peripheral nerve damage is associated with high long-term morbidity. Because of beneficial secretome, immunomodulatory effects, and ease of clinical translation, transplantation with adipose-derived stem cells (ASC) represents a promising therapeutic modality.METHODS:
Effect of ASC delivery in poloxamer hydrogel was assessed in a rat sciatic nerve model of critical-sized (1.5 cm) peripheral nerve injury. Nerve/muscle unit regeneration was assessed via immunostaining explanted nerve, quantitative polymerase chain reaction (qPCR), and histological analysis of reinnervating gastrocnemius muscle.RESULTS:
On the basis of viability data, 10% poloxamer hydrogel was selected for in vivo study. Six weeks after transection and repair, the group treated with poloxamer delivered ASCs demonstrated longest axonal regrowth. The qPCR results indicated that the inclusion of ASCs appeared to result in expression of factors that aid in reinnervating muscle tissue.DISCUSSION:
Delivery of ASCs in poloxamer addresses multiple facets of the complexity of nerve/muscle unit regeneration, representing a promising avenue for further study. Muscle Nerve 58 251-260, 2018.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nervos Periféricos
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Adipócitos
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Poloxâmero
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Hidrogéis
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Transplante de Células-Tronco
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Regeneração Nervosa
Tipo de estudo:
Prognostic_studies
Limite:
Adult
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Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article