Reactivity-driven cleanup of 2-Aminobenzamide derivatized oligosaccharides.
Anal Biochem
; 546: 23-27, 2018 04 01.
Article
em En
| MEDLINE
| ID: mdl-29407581
ABSTRACT
N-glycan profiling is commonly accomplished by the derivatization of the enzymatically released oligosaccharides with a fluorophore, thereby facilitating their analysis by hydrophilic-interaction liquid chromatography (HILIC). These fluorescent dyes are often present in large excess during derivatization reactions, and their removal is typically required to minimize chromatographic interference. Herein, we report a reactivity-driven 2-phase extraction protocol with the aldehyde reagent octanal, which demonstrated efficient 2-aminobenzamide cleanup as well as high derivatized N-glycan recovery. This cleanup method can be performed within minutes, and therefore provides an alternative sample preparation route for N-glycan profiling with improved time efficiency and operational simplicity.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Oligossacarídeos
/
Ortoaminobenzoatos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article