Cdk9 regulates a promoter-proximal checkpoint to modulate RNA polymerase II elongation rate in fission yeast.
Nat Commun
; 9(1): 543, 2018 02 07.
Article
em En
| MEDLINE
| ID: mdl-29416031
ABSTRACT
Post-translational modifications of the transcription elongation complex provide mechanisms to fine-tune gene expression, yet their specific impacts on RNA polymerase II regulation remain difficult to ascertain. Here, in Schizosaccharomyces pombe, we examine the role of Cdk9, and related Mcs6/Cdk7 and Lsk1/Cdk12 kinases, on transcription at base-pair resolution with Precision Run-On sequencing (PRO-seq). Within a minute of Cdk9 inhibition, phosphorylation of Pol II-associated factor, Spt5 is undetectable. The effects of Cdk9 inhibition are more severe than inhibition of Cdk7 and Cdk12, resulting in a shift of Pol II toward the transcription start site (TSS). A time course of Cdk9 inhibition reveals that early transcribing Pol II can escape promoter-proximal regions, but with a severely reduced elongation rate of only ~400 bp/min. Our results in fission yeast suggest the existence of a conserved global regulatory checkpoint that requires Cdk9 kinase activity.
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Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
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RNA Polimerase II
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Regiões Promotoras Genéticas
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Genes cdc
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Proteínas de Schizosaccharomyces pombe
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Fatores de Elongação da Transcrição
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Quinase 9 Dependente de Ciclina
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article