"NOTA-PRGD2 and NODAGA-PRGD2 : Bioconjugation, characterization, radiolabelling, and design space".
J Labelled Comp Radiopharm
; 61(6): 487-500, 2018 05 30.
Article
em En
| MEDLINE
| ID: mdl-29430693
ABSTRACT
This work reports on the development of amide bond bioconjugation for the production of -NOTA and -NODAGA PRGD2 using batch strategy and microfluidic reactor technology. The final radiolabelling step was fully optimized using Design of Experiments and Design Space approaches, hence targeting robust labelling yields in routine. Optimal labelling conditions were defined in sodium acetate buffer as 168 µg/mL peptide concentration, 4.9 pH, 47.5°C temperature, and 12.5-minute reaction time. Upon optimization, the Gallium-68 radiolabelling was fully automated. All the work was designed to be compliant to the GMP environment and to support the pharmaceutical scale-up.
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Base de dados:
MEDLINE
Assunto principal:
Oligopeptídeos
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Compostos Organometálicos
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Compostos Policíclicos
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Compostos Radiofarmacêuticos
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Amidas
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Radioisótopos de Gálio
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article