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Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis.
Biswal, Ajaya K; Atmodjo, Melani A; Li, Mi; Baxter, Holly L; Yoo, Chang Geun; Pu, Yunqiao; Lee, Yi-Ching; Mazarei, Mitra; Black, Ian M; Zhang, Ji-Yi; Ramanna, Hema; Bray, Adam L; King, Zachary R; LaFayette, Peter R; Pattathil, Sivakumar; Donohoe, Bryon S; Mohanty, Sushree S; Ryno, David; Yee, Kelsey; Thompson, Olivia A; Rodriguez, Miguel; Dumitrache, Alexandru; Natzke, Jace; Winkeler, Kim; Collins, Cassandra; Yang, Xiaohan; Tan, Li; Sykes, Robert W; Gjersing, Erica L; Ziebell, Angela; Turner, Geoffrey B; Decker, Stephen R; Hahn, Michael G; Davison, Brian H; Udvardi, Michael K; Mielenz, Jonathan R; Davis, Mark F; Nelson, Richard S; Parrott, Wayne A; Ragauskas, Arthur J; Neal Stewart, C; Mohnen, Debra.
Afiliação
  • Biswal AK; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
  • Atmodjo MA; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.
  • Li M; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Baxter HL; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
  • Yoo CG; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.
  • Pu Y; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Lee YC; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Mazarei M; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Black IM; UT-ORNL Joint Institute for Biological Sciences, Oak Ridge, Tennessee, USA.
  • Zhang JY; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Ramanna H; Department of Plant Sciences, University of Tennessee, Knoxville, Tennessee, USA.
  • Bray AL; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • King ZR; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • LaFayette PR; UT-ORNL Joint Institute for Biological Sciences, Oak Ridge, Tennessee, USA.
  • Pattathil S; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Donohoe BS; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Mohanty SS; UT-ORNL Joint Institute for Biological Sciences, Oak Ridge, Tennessee, USA.
  • Ryno D; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Yee K; Noble Research Institute, Ardmore, Oklahoma, USA.
  • Thompson OA; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Rodriguez M; Department of Plant Sciences, University of Tennessee, Knoxville, Tennessee, USA.
  • Dumitrache A; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.
  • Natzke J; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Winkeler K; Noble Research Institute, Ardmore, Oklahoma, USA.
  • Collins C; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Yang X; Noble Research Institute, Ardmore, Oklahoma, USA.
  • Tan L; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Sykes RW; Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, Georgia, USA.
  • Gjersing EL; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Ziebell A; Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, Georgia, USA.
  • Turner GB; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Decker SR; Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, Georgia, USA.
  • Hahn MG; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.
  • Davison BH; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Udvardi MK; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Mielenz JR; National Renewable Energy Laboratory, Golden, Colorado, USA.
  • Davis MF; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.
  • Nelson RS; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Parrott WA; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.
  • Ragauskas AJ; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Neal Stewart C; DOE-BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
  • Mohnen D; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
Nat Biotechnol ; 36(3): 249-257, 2018 03.
Article em En | MEDLINE | ID: mdl-29431741
ABSTRACT
Cell walls in crops and trees have been engineered for production of biofuels and commodity chemicals, but engineered varieties often fail multi-year field trials and are not commercialized. We engineered reduced expression of a pectin biosynthesis gene (Galacturonosyltransferase 4, GAUT4) in switchgrass and poplar, and find that this improves biomass yields and sugar release from biomass processing. Both traits were maintained in a 3-year field trial of GAUT4-knockdown switchgrass, with up to sevenfold increased saccharification and ethanol production and sixfold increased biomass yield compared with control plants. We show that GAUT4 is an α-1,4-galacturonosyltransferase that synthesizes homogalacturonan (HG). Downregulation of GAUT4 reduces HG and rhamnogalacturonan II (RGII), reduces wall calcium and boron, and increases extractability of cell wall sugars. Decreased recalcitrance in biomass processing and increased growth are likely due to reduced HG and RGII cross-linking in the cell wall.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Parede Celular / Pectinas / Glucuronosiltransferase / Biocombustíveis Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Parede Celular / Pectinas / Glucuronosiltransferase / Biocombustíveis Idioma: En Ano de publicação: 2018 Tipo de documento: Article