Nonribosomal biosynthesis of backbone-modified peptides.
Nat Chem
; 10(3): 282-287, 2018 03.
Article
em En
| MEDLINE
| ID: mdl-29461527
ABSTRACT
Biosynthetic modification of nonribosomal peptide backbones represents a potentially powerful strategy to modulate the structure and properties of an important class of therapeutics. Using a high-throughput assay for catalytic activity, we show here that an L-Phe-specific module of an archetypal nonribosomal peptide synthetase can be reprogrammed to accept and process the backbone-modified amino acid (S)-ß-Phe with near-native specificity and efficiency. A co-crystal structure with a non-hydrolysable aminoacyl-AMP analogue reveals the origins of the 40,000-fold α/ß-specificity switch, illuminating subtle but precise remodelling of the active site. When the engineered catalyst was paired with downstream module(s), (S)-ß-Phe-containing peptides were produced at preparative scale in vitro (~1â
mmol) and high titres in vivo (~100â
mgâ
l-1), highlighting the potential of biosynthetic pathway engineering for the construction of novel nonribosomal ß-frameworks.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Biossíntese Peptídica
/
Peptídeo Sintases
/
Peptídeos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article