Human AK2 links intracellular bioenergetic redistribution to the fate of hematopoietic progenitors.
Biochem Biophys Res Commun
; 497(2): 719-725, 2018 03 04.
Article
em En
| MEDLINE
| ID: mdl-29462620
ABSTRACT
AK2 is an adenylate phosphotransferase that localizes at the intermembrane spaces of the mitochondria, and its mutations cause a severe combined immunodeficiency with neutrophil maturation arrest named reticular dysgenesis (RD). Although the dysfunction of hematopoietic stem cells (HSCs) has been implicated, earlier developmental events that affect the fate of HSCs and/or hematopoietic progenitors have not been reported. Here, we used RD-patient-derived induced pluripotent stem cells (iPSCs) as a model of AK2-deficient human cells. Hematopoietic differentiation from RD-iPSCs was profoundly impaired. RD-iPSC-derived hemoangiogenic progenitor cells (HAPCs) showed decreased ATP distribution in the nucleus and altered global transcriptional profiles. Thus, AK2 has a stage-specific role in maintaining the ATP supply to the nucleus during hematopoietic differentiation, which affects the transcriptional profiles necessary for controlling the fate of multipotential HAPCs. Our data suggest that maintaining the appropriate energy level of each organelle by the intracellular redistribution of ATP is important for controlling the fate of progenitor cells.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Hematopoéticas
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Trifosfato de Adenosina
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Adenilato Quinase
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Imunodeficiência Combinada Severa
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Células-Tronco Pluripotentes Induzidas
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Hematopoese
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Leucopenia
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article