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Hunting for cultivable Micromonospora strains in soils of the Atacama Desert.
Carro, Lorena; Razmilic, Valeria; Nouioui, Imen; Richardson, Lee; Pan, Che; Golinska, Patrycja; Asenjo, Juan A; Bull, Alan T; Klenk, Hans-Peter; Goodfellow, Michael.
Afiliação
  • Carro L; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, UK. lcg@usal.es.
  • Razmilic V; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, UK.
  • Nouioui I; Department of Chemical Engineering and Biotechnology, Centre for Biotechnology and Bioengineering (CeBiB), Universidad de Chile, Beauchef 850, Santiago, Chile.
  • Richardson L; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, UK.
  • Pan C; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, UK.
  • Golinska P; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, UK.
  • Asenjo JA; Department of Microbiology, Nicolaus Copernicus University, Torun, Poland.
  • Bull AT; Department of Chemical Engineering and Biotechnology, Centre for Biotechnology and Bioengineering (CeBiB), Universidad de Chile, Beauchef 850, Santiago, Chile.
  • Klenk HP; School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ, UK.
  • Goodfellow M; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, UK.
Antonie Van Leeuwenhoek ; 111(8): 1375-1387, 2018 Aug.
Article em En | MEDLINE | ID: mdl-29480426
ABSTRACT
Innovative procedures were used to selectively isolate small numbers of Micromonospora strains from extreme hyper-arid and high altitude Atacama Desert soils. Micromonosporae were recognised on isolation plates by their ability to produce filamentous microcolonies that were strongly attached to the agar. Most of the isolates formed characteristic orange colonies that lacked aerial hyphae and turned black on spore formation, whereas those from the high altitude soil were dry, blue-green and covered by white aerial hyphae. The isolates were assigned to seven multi- and eleven single-membered groups based on BOX-PCR profiles. Representatives of the groups were assigned to either multi-membered clades that also contained marker strains or formed distinct phyletic lines in the Micromonospora 16S rRNA gene tree; many of the isolates were considered to be putatively novel species of Micromonospora. Most of the isolates from the high altitude soils showed activity against wild type strains of Bacillus subtilis and Pseudomonas fluorescens while those from the rhizosphere of Parastrephia quadrangulares and from the Lomas Bayas hyper-arid soil showed resistance to UV radiation.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Filogenia / Microbiologia do Solo / Clima Desértico / Micromonospora País como assunto: America do sul / Chile Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Filogenia / Microbiologia do Solo / Clima Desértico / Micromonospora País como assunto: America do sul / Chile Idioma: En Ano de publicação: 2018 Tipo de documento: Article