Comparison between iMSD and 2D-pCF analysis for molecular motion studies on in vivo cells: The case of the epidermal growth factor receptor.
Methods
; 140-141: 74-84, 2018 05 01.
Article
em En
| MEDLINE
| ID: mdl-29501424
ABSTRACT
Image correlation analysis has evolved to become a valuable method of analysis of the diffusional motion of molecules in every points of a live cell. Here we compare the iMSD and the 2D-pCF approaches that provide complementary information. The iMSD method provides the law of diffusion and it requires spatial averaging over a small region of the cell. The 2D-pCF does not require spatial averaging and it gives information about obstacles for diffusion at pixel resolution. We show the analysis of the same set of data by the two methods to emphasize that both methods could be needed to have a comprehensive understanding of the molecular diffusional flow in a live cell.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
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Microscopia Intravital
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Microscopia de Fluorescência
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Modelos Químicos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article