Ultrafast photophysics of the environment-sensitive 4'-methoxy-3-hydroxyflavone fluorescent dye.
Phys Chem Chem Phys
; 20(11): 7885-7895, 2018 Mar 14.
Article
em En
| MEDLINE
| ID: mdl-29509200
ABSTRACT
The excited state intramolecular proton transfer (ESIPT) of 3-hydroxyflavone derivatives results in a fluorescence spectrum composed of two emission bands, the relative intensity of which is strongly influenced by the interaction with the local environment. We use time-resolved fluorescence and ultrafast transient absorption spectroscopies to investigate the photophysics of 4'-methoxy-3-hydroxyflavone in different solvents characterized by various polarities and hydrogen (H) bonding capabilities. We evidence that in this compound, the ESIPT reaction rate varies by more than 3 orders of magnitude, depending on the H-bonding capability of its local environment. This remarkable property is attributed to the moderate electron-donating strength of the 4'-methoxy substituent, and turns this fluorescent dye into a very promising fluorescent probe of biomolecular structures and interactions, where local structural heterogeneity may possibly be revealed by resolving a distribution of ESIPT reaction rates.
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Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article