MicroRNA4284 promotes gastric cancer tumorigenicity by targeting ten-eleven translocation 1.
Mol Med Rep
; 17(5): 6569-6575, 2018 05.
Article
em En
| MEDLINE
| ID: mdl-29512746
ABSTRACT
Increasing evidence has shown that abnormal expression of miR-4284 participates in the progression of several types of cancer. However, the expression and the role of miR4284 in gastric cancer remain largely unknown. Therefore, in the present study the miR4284 expression levels in gastric cancer tissues and cell lines, was examined using reverse transcriptionquantitative polymerase chain reaction (RTqPCR) and found that miR4284 was significantly upregulated in 40 pairs of gastric cancer tissues and five gastric cancer cell lines compared to the corresponding normal tissues and GES1 cell line. In addition, increased miR4284 expression was positively associated with TNM stage (P=0.035), distal metastasis (P=0.022) and poor prognosis in gastric cancer patients. Furthermore, the overexpression of miR4284 expression was shown to promote cell proliferation, clone formation, invasion and migration, while the suppression of miR4284 expression induced opposite effects. Additionally, luciferase reporter assay was conducted and showed that ten-eleven translocation 1 (TET1), a tumor suppressor gene that regulating cell survival and metastasis, was a direct target of miR4284. Upregulated miR4284 decreased the mRNA and protein levels of TET1 in SGC7901 cells and downregulated miR4284 increased the mRNA and protein levels of TET1 in AGS cells. In addition, miR4284 expression was negatively correlated with the TET1 expression in gastric cancer tissues. Moreover, inhibition of TET1 suppressed the effect of miR4284 inhibitors on cell proliferation in AGS cells. Therefore, data demonstrated that miR4284 could promote tumor cell growth, migration and invasion by directly targeting TET1 in gastric cancer, which may provide a potential therapeutic target for gastric cancer treatment.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Gástricas
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RNA Neoplásico
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Regulação Enzimológica da Expressão Gênica
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Regulação Neoplásica da Expressão Gênica
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Movimento Celular
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Proteínas Proto-Oncogênicas
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MicroRNAs
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Oxigenases de Função Mista
Limite:
Female
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Humans
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Male
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article