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PCMT1 is an unfavorable predictor and functions as an oncogene in bladder cancer.
Dong, Liming; Li, Yanpei; Xue, Dongwei; Liu, Yili.
Afiliação
  • Dong L; Department of Urology, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan Road East, Huanggu District, Liaoning, Shenyang, China.
  • Li Y; Department of Urology, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan Road East, Huanggu District, Liaoning, Shenyang, China.
  • Xue D; Department of Urology, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan Road East, Huanggu District, Liaoning, Shenyang, China.
  • Liu Y; Department of Urology, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan Road East, Huanggu District, Liaoning, Shenyang, China.
IUBMB Life ; 70(4): 291-299, 2018 04.
Article em En | MEDLINE | ID: mdl-29517839
ABSTRACT
The role of protein l-isoaspartate (d-aspartate) O-methyltransferase (PCMT1) in human cancer was generally cognized. The clinical significance and biological function of PCMT1 in bladder cancer is still unknown. PCMT1 mRNA and protein expression levels in bladder cancer tissues and cell lines were detected by qRT-PCR, immunohistochemistry, or western blot. The correlation between PCMT1 expression and clinicopathological factors was analyzed through immunohistochemistry in 108 bladder cancer patients. Loss-of-function and gain-of-function studies were conducted to explore the biological function of PCMT1 in bladder cancer cell lines in regulating cell proliferation, migration, and invasion. In our results, we found that PCMT1 was overexpressed in bladder cancer tissues compared with normal urothelium tissues in microarray datasets (GSE3167). Then, we confirmed PCMT1 mRNA and protein expression were increased in bladder cancer tissues and cell lines compared with paired normal urothelium tissues and normal uroepithelial cell line. PCMT1 protein expression was obviously correlated with clinical stage, muscularis invasion, lymph node metastasis, and distant metastasis. Survival analysis showed that PCMT1 protein high-expression was an independent unfavorable prognostic factor for bladder cancer patients. The in vitro experiments showed PCMT1 regulated bladder cancer cells migration and invasion through modulating epithelial-mesenchymal transition (EMT)-associated genes expression including E-cadherin, vimentin, Snail and Slug, but had no effect on proliferation. In conclusion, PCMT1 is an unfavorable prognostic biomarker and involves in cells migration and invasion through regulating EMT-associated genes. © 2018 IUBMB Life, 70(4)291-299, 2018.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Bexiga Urinária / Biomarcadores Tumorais / Proteína D-Aspartato-L-Isoaspartato Metiltransferase / Transição Epitelial-Mesenquimal Tipo de estudo: Observational_studies / Prognostic_studies Limite: Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Bexiga Urinária / Biomarcadores Tumorais / Proteína D-Aspartato-L-Isoaspartato Metiltransferase / Transição Epitelial-Mesenquimal Tipo de estudo: Observational_studies / Prognostic_studies Limite: Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2018 Tipo de documento: Article