Cationic polymers for non-viral gene delivery to human T cells.
J Control Release
; 282: 140-147, 2018 07 28.
Article
em En
| MEDLINE
| ID: mdl-29518467
ABSTRACT
The clinical success of chimeric antigen receptor (CAR) T cell immunotherapy in treating multiple blood cancers has created a need for efficient methods of ex vivo gene delivery to primary human T cells for cell engineering. Here, we synthesize and evaluate a panel of cationic polymers for gene delivery to both cultured and primary human T cells. We show that a subset of comb- and sunflower-shaped pHEMA-g-pDMAEMA polymers can mediate transfection with efficiencies up to 50% in the Jurkat human T cell line with minimal concomitant toxicity (>90% viability). We then optimize primary human T cell transfection conditions including activation time, cell density, DNA dose, culture media, and cytokine treatment. We demonstrate transfection of both CD4+ and CD8+ primary human T cells with messenger RNA and plasmid DNA at efficiencies up to 25 and 18%, respectively, with similarly high viability.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Poli-Hidroxietil Metacrilato
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DNA
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RNA Mensageiro
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Portadores de Fármacos
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Linfócitos T
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Transfecção
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Metacrilatos
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Nylons
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article