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Detection of amyloid fibrils in Parkinson's disease using plasmonic chirality.
Kumar, Jatish; Eraña, Hasier; López-Martínez, Elena; Claes, Nathalie; Martín, Víctor F; Solís, Diego M; Bals, Sara; Cortajarena, Aitziber L; Castilla, Joaquín; Liz-Marzán, Luis M.
Afiliação
  • Kumar J; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), 20014 Donostia-San Sebastian, Spain.
  • Eraña H; Center for Cooperative Research in Biosciences (CIC bioGUNE), 48160 Derio, Bizkaia, Spain.
  • López-Martínez E; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), 20014 Donostia-San Sebastian, Spain.
  • Claes N; Electron Microscopy for Materials Science (EMAT), University of Antwerp, B-2020 Antwerp, Belgium.
  • Martín VF; Departamento de Tecnología de los Computadores y de las Comunicaciones, University of Extremadura, 10003 Cáceres, Spain.
  • Solís DM; Departamento de Teoría de la Señal y Comunicaciones, University of Vigo, 36301 Vigo, Spain.
  • Bals S; Electron Microscopy for Materials Science (EMAT), University of Antwerp, B-2020 Antwerp, Belgium.
  • Cortajarena AL; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), 20014 Donostia-San Sebastian, Spain.
  • Castilla J; Ikerbasque, Basque Foundation for Science, 48013 Bilbao, Spain.
  • Liz-Marzán LM; Center for Cooperative Research in Biosciences (CIC bioGUNE), 48160 Derio, Bizkaia, Spain.
Proc Natl Acad Sci U S A ; 115(13): 3225-3230, 2018 03 27.
Article em En | MEDLINE | ID: mdl-29531058
Amyloid fibrils, which are closely associated with various neurodegenerative diseases, are the final products in many protein aggregation pathways. The identification of fibrils at low concentration is, therefore, pivotal in disease diagnosis and development of therapeutic strategies. We report a methodology for the specific identification of amyloid fibrils using chiroptical effects in plasmonic nanoparticles. The formation of amyloid fibrils based on α-synuclein was probed using gold nanorods, which showed no apparent interaction with monomeric proteins but effective adsorption onto fibril structures via noncovalent interactions. The amyloid structure drives a helical nanorod arrangement, resulting in intense optical activity at the surface plasmon resonance wavelengths. This sensing technique was successfully applied to human brain homogenates of patients affected by Parkinson's disease, wherein protein fibrils related to the disease were identified through chiral signals from Au nanorods in the visible and near IR, whereas healthy brain samples did not exhibit any meaningful optical activity. The technique was additionally extended to the specific detection of infectious amyloids formed by prion proteins, thereby confirming the wide potential of the technique. The intense chiral response driven by strong dipolar coupling in helical Au nanorod arrangements allowed us to detect amyloid fibrils down to nanomolar concentrations.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doença de Parkinson / Nanotubos / Alfa-Sinucleína / Amiloide Tipo de estudo: Diagnostic_studies Limite: Aged / Female / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doença de Parkinson / Nanotubos / Alfa-Sinucleína / Amiloide Tipo de estudo: Diagnostic_studies Limite: Aged / Female / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article