An antibody-free assay for simultaneous capture and detection of glycoproteins by surface enhanced Raman spectroscopy.
Phys Chem Chem Phys
; 20(13): 8881-8886, 2018 Mar 28.
Article
em En
| MEDLINE
| ID: mdl-29546894
ABSTRACT
A sensitive, and single step reaction assay of glycoproteins in antibody-free mode was proposed on a 4-mercapto-phenylboronic acid (4-MPBA) modified silver nanoparticle film (AgNF) by using surface enhanced Raman scattering (SERS). 4-MPBA/AgNF was designed both as a glycoprotein-specific recognition biosensor and as a Raman reporter for the SERS platform. Variation in the ratio I1574/I1586 for 4-MPBA/AgNF in response to glycoprotein capture constitutes the SERS assay which exhibits high sensitivity and selectivity against nonglycoproteins and is shown to function in complex samples.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Glicoproteínas
/
Técnicas de Química Analítica
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article