A ketogenic amino acid rich diet benefits mitochondrial homeostasis by altering the AKT/4EBP1 and autophagy signaling pathways in the gastrocnemius and soleus.
Biochim Biophys Acta Gen Subj
; 1862(7): 1547-1555, 2018 Jul.
Article
em En
| MEDLINE
| ID: mdl-29550428
ABSTRACT
Muscle biology is important topic in diabetes research. We have reported that a diet with ketogenic amino acids rich replacement (KAAR) ameliorated high-fat diet (HFD)-induced hepatosteatosis via activation of the autophagy system. Here, we found that a KAAR ameliorated the mitochondrial morphological alterations and associated mitochondrial dysfunction induced by an HFD through induction of the AKT/4EBP1 and autophagy signaling pathways in both fast and slow muscles. The mice were fed with a standard HFD (30% fat in food) or an HFD with KAAR (HFDKAAR). In both the gastrocnemius and the soleus, HFDKAAR ameliorated HFD-impaired mitochondrial morphology and mitochondrial function, characterized by decreased mitofusin 2, optic atrophy 1, peroxisome proliferator-activated receptor (PPAR) γ coactivator-1α and PPARα levels and increased dynamin-related protein 1 levels. The decreased levels of phosphorylated AKT and 4EBP1 in the gastrocnemius and soleus of HFD-fed mice were remediated by HFDKAAR. Furthermore, the HFDKAAR ameliorated the HFD-induced autophagy defects in the gastrocnemius and soleus. These findings suggest that KAAR may be a novel strategy to combat obesity-induced mitochondrial dysfunction, likely through induction of the AKT/4EBP1 and autophagy pathways in skeletal muscle.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
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Autofagia
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Transdução de Sinais
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Proteínas de Transporte
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Músculo Esquelético
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Proteínas Proto-Oncogênicas c-akt
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Dieta Cetogênica
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Aminoácidos
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Mitocôndrias
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Obesidade
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article