miR-503 suppresses the proliferation and metastasis of esophageal squamous cell carcinoma by triggering autophagy via PKA/mTOR signaling.
Int J Oncol
; 52(5): 1427-1442, 2018 May.
Article
em En
| MEDLINE
| ID: mdl-29568867
ABSTRACT
MicroRNA (miR)-503 is involved in the regulation of the malignant phenotype in multiple tumor types, and has been proven to be a novel diagnostic and therapeutic target; however, its function and mechanisms of action have not yet been fully elucidated in esophageal squamous cell carcinoma (ESCC). In the current study, we detected miR503 expression by RTqPCR and found that miR503 expression was increased in ESCC, but negatively correlated with lymph node metastasis, TNM stage and tumor differentiation. Functionally, we confirmed that miR503 inhibited the proliferation and metastasis of ESCC cells by triggering cellular autophagy. Mechanistically, we confirmed that miR503 exerted its biological effects by targeting protein kinase CAMPactivated catalytic subunit alpha (PRKACA) in ESCC by dual luciferase reporter assay. Moreover, miR503 was found to trigger autophagy in ESCC cells through the protein kinase A (PKA)/mammalian target of rapamycin (mTOR) pathway. Taken together, our results demonstrate that miR503 suppresses the proliferation and metastasis of ESCC via the activation of autophagy, mediated by the PKA/mTOR signaling pathway.
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MEDLINE
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En
Ano de publicação:
2018
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Article