Higher susceptibility to osmolality of the medaka (Oryzias latipes) mutants in orthologue genes of mammalian skin transglutaminases.

Watanabe, Yuko; Furukawa, Eri; Tatsukawa, Hideki; Hashimoto, Hisashi; Kamei, Yasuhiro; Taniguchi, Yoshihito; Hitomi, Kiyotaka

Watanabe, Yuko; Furukawa, Eri; Tatsukawa, Hideki; Hashimoto, Hisashi; Kamei, Yasuhiro; Taniguchi, Yoshihito; Hitomi, Kiyotaka.

Afiliação

Watanabe Y; a Graduate School of Pharmaceutical Sciences , Nagoya University , Nagoya , Japan.
Furukawa E; a Graduate School of Pharmaceutical Sciences , Nagoya University , Nagoya , Japan.
Tatsukawa H; a Graduate School of Pharmaceutical Sciences , Nagoya University , Nagoya , Japan.
Hashimoto H; b Bioscience Biotechnology Center Nagoya University , Nagoya , Japan.
Kamei Y; c National Institute for Basic Biology , Okazaki , Japan.
Taniguchi Y; d Department of Preventive Medicine and Public Health, School of Medicine , Kyorin University , Tokyo , Japan.
Hitomi K; a Graduate School of Pharmaceutical Sciences , Nagoya University , Nagoya , Japan.

Biosci Biotechnol Biochem ; 82(7): 1165-1168, 2018 Jul.

Article em En | MEDLINE | ID: mdl-29570037

ABSTRACT

ABSTRACT

Transglutaminase (TG) is an essential enzyme to catalyze cross-linking reactions of epidermal proteins. Recently, we biochemically characterized human skin TG orthologues for medaka (Oryzias latipes), a model fish. By genome editing, gene-modified fishes for the two orthologues were obtained, both of which lack the ordinal enzymes. These fish appeared to exhibit higher susceptibility to osmolality at the period of larvae.

Assuntos

Proteínas de Peixes/genética; Mutação; Oryzias/fisiologia; Concentração Osmolar; Pele/enzimologia; Transglutaminases/genética; Sequência de Aminoácidos; Animais; Animais Geneticamente Modificados; Feminino; Edição de Genes; Humanos; Larva/fisiologia; Masculino; Oryzias/genética; Oryzias/crescimento & desenvolvimento; Transglutaminases/química

Palavras-chave

KO: knockout; TG: transglutaminase; Tilling: Targeting-induced local lesions in genome method; Transglutaminase; WT: wild-type; medaka; skin

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Concentração Osmolar / Pele / Oryzias / Transglutaminases / Proteínas de Peixes / Mutação Limite: Animals / Female / Humans / Male Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google