Expression of heterologous lycopene ß-cyclase gene in flax can cause silencing of its endogenous counterpart by changes in gene-body methylation and in ABA homeostasis mechanism.
Plant Physiol Biochem
; 127: 143-151, 2018 Jun.
Article
em En
| MEDLINE
| ID: mdl-29579641
ABSTRACT
Previously we described flax plants with expression of Arabidopsis lycopene ß-cyclase (lcb) gene in which decreased expression of the endogenous lcb and increased resistance to fungal pathogen was observed. We suggested that co-suppression was responsible for the change. In this study we investigated the molecular basis of the observed effect in detail. We found that methylation changes in the Lulcb gene body might be responsible for repression of the gene. Treatment with azacitidine (DNA methylation inhibitor) confirmed the results. Moreover, we studied how the manipulation of carotenoid biosynthesis pathway increased ABA level in these plants. We suggest that elevated ABA levels may be responsible for the increased resistance of the flax plants to pathogen infection through activation of chitinase (PR gene).
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Base de dados:
MEDLINE
Assunto principal:
Regulação Enzimológica da Expressão Gênica
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Ácido Abscísico
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Regulação da Expressão Gênica de Plantas
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DNA de Plantas
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Metilação de DNA
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Linho
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Liases Intramoleculares
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Inativação Gênica
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article